Active-site structural comparison of streptococcal NADH peroxidase and NADH oxidase. Reconstitution with artificial flavins.
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چکیده
منابع مشابه
Cofactor Recycling Using a Thermostable NADH Oxidase
From the standpoint of enzymatic organic synthesis, NADH oxidase (NOX) will be a key enzyme that plays an essential role in the cofactor regeneration of NAD+ dependent enzymatic reactions. For example, enzymatic enantioselective oxidations of racemic secondary alcohols (Geueke et al., 2003; Riebel et al., 2003; Hummel & Riebel, 1996) and amino acids (Hummel et al., 2003a) have been reported as ...
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Flavin enzymes are unique as biological catalysts in that they carry out a wide variety of different biochemical processes. This sets them apart from enzymes which use other coenzymes, like pyridine nucleotides, pyridoxal phosphate or thiamin pyrophosphate, each of which involves a single type of chemical event. Flavins thus are involved in the dehydrogenation of a variety of substrates, in one...
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Growth of 12 Leuconostoc strains in a broth medium with and without aeration was compared. In general, the aerated cultures grew faster and produced more biomass, at the expense of glucose and other sugars, than unaerated cultures. The more efficient growth correlated well with the production of acetate rather than ethanol as an end-product of metabolism in aerated cultures; unaerated cultures ...
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Peroxidases (POD) and polyphenol oxidase (PPO) are enzymes that are well known to be involved in the enzymatic browning reaction of fruits and vegetables with different catalytic mechanisms. Both enzymes have some common substrates, but each also has its specific substrates. In our computational study, the amino acid sequence of grape peroxidase (ABX) was used for the construction of models emp...
متن کاملPurification and characterization of NADH oxidase from Serpulina (Treponema) hyodysenteriae.
NADH oxidase (EC 1.6.99.3) was purified from cell lysates of Serpulina (Treponema) hyodysenteriae B204 by differential ultracentrifugation, ammonium sulfate precipitation, and chromatography on anion-exchange, dye-ligand-affinity, and size-exclusion columns. Purified NADH oxidase had a specific activity 119-fold higher than that of cell lysates and migrated as a single band during denaturing ge...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)50601-9